LymphedemaV1, Main, Exploration, bibRecord, 00D222

Isozymic pattern of lactate dehydrogenase in cases of bancroftian filariasis

Identifieur interne : 00D222 ( Main/Exploration ); précédent : 00D221; suivant : 00D223

Isozymic pattern of lactate dehydrogenase in cases of bancroftian filariasis

Auteurs : S. Misra [Inde] ; T. M. Mahopatra [Inde] ; S. Rathaur [Inde]

Source :

Journal of Helminthology [ 0022-149X ] ; 1992-06.

RBID : ISTEX:D685FB359825C80921357EE2CD015C582F6B35AF

Descripteurs français

KwdFr :
- Animaux, Filariose lymphatique (enzymologie), Humains, Isoenzymes, L-Lactate dehydrogenase (sang), Wuchereria bancrofti, Électrophorèse sur gel de polyacrylamide.
MESH :
- enzymologie : Filariose lymphatique.
- sang : L-Lactate dehydrogenase.
- Animaux, Humains, Isoenzymes, Wuchereria bancrofti, Électrophorèse sur gel de polyacrylamide.

English descriptors

KwdEn :
- Animals, Electrophoresis, Polyacrylamide Gel, Elephantiasis, Filarial (enzymology), Humans, Isoenzymes, L-Lactate Dehydrogenase (blood), Wuchereria bancrofti.
MESH :
- chemical , blood : L-Lactate Dehydrogenase.
- chemical : Isoenzymes.
- enzymology : Elephantiasis, Filarial.
- Animals, Electrophoresis, Polyacrylamide Gel, Humans, Wuchereria bancrofti.

Abstract

Isozymic patterns of lactate dehydrogenase (E.C. 1.1.1.27) by polyacrylamide gel electrophoresis (PAGE) were observed m various categories of filariasis and controls, i.e. asymptomatic microfilaraemia and symptomatic amicrofilaraemia, endemic normal and non-endemic normal. Lactate dehydrogenase (LDH) activity was also observed amongst the above categories of patients. An increase in enzyme activity and change in the isozymic pattern was observed in the above categories of filaria infected serum. LDH activity doubled in asymptomatic microfilaraemia whereas in symptomatic amicrofilaraemia the increase in LDH activity was thirtyfold. The isozymic pattern of microfilaraemia cases showed the presence of three bands B4, A1B3, A2 B2, which are quite thick as compared to normal healthy subjects, whereas the patients with symptomatic amicrofilaraemia showed marked elevation of serum LDH-4 or A3B1. The LDH was partially purified by combined treatment of (NH4)2SO4 fractionation and gel filtration. The isozymic pattern of purified LDH showed a similar pattern.

Url:

https://api.istex.fr/document/D685FB359825C80921357EE2CD015C582F6B35AF/fulltext/pdf

DOI: 10.1017/S0022149X00012736

Affiliations:

Inde

Le document en format XML

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<front><div type="abstract">Isozymic patterns of lactate dehydrogenase (E.C. 1.1.1.27) by polyacrylamide gel electrophoresis (PAGE) were observed m various categories of filariasis and controls, i.e. asymptomatic microfilaraemia and symptomatic amicrofilaraemia, endemic normal and non-endemic normal. Lactate dehydrogenase (LDH) activity was also observed amongst the above categories of patients. An increase in enzyme activity and change in the isozymic pattern was observed in the above categories of filaria infected serum. LDH activity doubled in asymptomatic microfilaraemia whereas in symptomatic amicrofilaraemia the increase in LDH activity was thirtyfold. The isozymic pattern of microfilaraemia cases showed the presence of three bands B4, A1B3, A2 B2, which are quite thick as compared to normal healthy subjects, whereas the patients with symptomatic amicrofilaraemia showed marked elevation of serum LDH-4 or A3B1. The LDH was partially purified by combined treatment of (NH4)2SO4 fractionation and gel filtration. The isozymic pattern of purified LDH showed a similar pattern.</div>
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Serveur d'exploration sur le lymphœdème

Isozymic pattern of lactate dehydrogenase in cases of bancroftian filariasis

Isozymic pattern of lactate dehydrogenase in cases of bancroftian filariasis

Source :

Descripteurs français

English descriptors

Abstract

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Pour manipuler ce document sous Unix (Dilib)

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